Research - Arsenicum album
ANTIOXIDANTE EFFECT IN VITRO OF THE HOMEOPATHIC MEDICINE ARSENICUM ALBUM, CUPRUM METALLICUM MANGANUM AND ZINCUM METALLICUM.
CELSO FERNANDES BATELLO
Dissertation presented as part of the demanded requirements to obtain the title of MA in Homeopathy by FACIS – IBEHE
Abstract
This dissertation, with the support of a theoretical practical foundation, presents the scenario where is inserted the problematic in question; The Antioxidant Effect in vitro of Homeopathic Medicines Arsenicum album, Cuprum metallicum, Manganum and Zincum metallicum.
It is demonstrated in the first chapters, of theoretical bibliographical substantiation, the Homeopathy and Oligotherapy as therapeutics techniques, as well as the importance of oxidation phenomena for a better comprehension of the organic phenomena, mainly in the genesis of many diseases. It is also experimentally demonstrated the homeopathic medicines antioxidant action in different dilutions in comparison with melatonin in various concentrations over the lipidic peroxidation in homogenate of mice brains measured though the malondialdehyde dosage obtained through the absorbency technique.
For the analysis of the results the Kruskal-Wallys and Dunn´s Multiple Comparisons tests were realized, that revealed significant differences among the experimented groups.
It was verified a greater lipidic peroxidation inhibiting effect with melatonin 1M, followed by melatonin 0.5M, Cuprum metallicum C12, Cuprum Metallicum C30, Arsenicum album C30, melatonine 0.24M, Manganum C30 and Arsenicum album C12.
It was proved that the melatonin has an in vitro lipidic peroxidation inhibiting effect, and so being adopted as reference. However the new fact arises from the observation of the significant lipidic peroxidation inhibition obtained with the usage of Homeopathic medicines, sometimes with dilutions that supersed the Avogadro number, as in the cases of Cuprum metallicum C30, Arsenicum album C30 and Manganum C30 in decreasing action order.
This work calls the attention for the possibility of existence of an antioxidant mechanism action of homeopathic medicine different from the know cause effect relationship.
Source : Homeopathy International
Link to Full Dissertation
Therapeutic Effect of Arsenicum album on Leukocytes
Elaine C. Ive1 , Ingrid M. S. Couchman1 and Lalini Reddy2,*
1 Department of Homoeopathy, Faculty of Health Sciences, Durban University of Technology, P.O. Box 1334, Durban 4000, South Africa
2 Department of Biotechnology and Food Technology, Faculty of Applied Sciences, Durban University of Technology, P.O. Box 1334, Durban 4000, South Africa
Abstract
The therapeutic effects of homoeopathic Arsenicum album potencies were investigated in-vitro, using a continuous cell line (MT4), pre-intoxicated with arsenic trioxide (As2O3), and then treated with succussed and unsuccussed homoeopathic potencies, 6CH, 30CH and 200CH. This study aimed to verify the homoeopathic law of similars and to determine whether potencies diluted beyond Avogadro’s constant had physiological effects on cells; whether various potencies would cause different effects as suggested by the concept of hormesis; whether succussed and unsuccussed homoeopathic potencies had different effects on the cells; and to establish whether a biotechnological method could be used to evaluate the above. As2O3 was used to pre-intoxicate and the MTT assay was used to measure the percentage cytotoxicity and half maximal inhibitory concentration (IC50) of the cells. The homoeopathic potencies of Arsenicum album (6CH, 30CH and 200CH) were prepared by either succussing or allowing to diffuse for 30 s. After pre-intoxication of the MT4 cells with the IC50 As2O3 and treatment with succussed and unsuccussed Arsenicum album (6CH-200CH), the cell viability increased with increasing potency from 81% to 194% (over 72 h). The treatments and the times of exposure were found to be statistically significant determinants of cell viability, whereas succussion did not cause any significant variation in the results. The study provided evidence that a biotechnological method (namely cell viability) may be used to scientifically evaluate the physiological effects of homoeopathic potencies on human cells; it confirmed that the homoeopathic potencies did have therapeutic effects; and that succussion was not required in the potentization method in order to produce a curative remedy.
Source : Int. J. Mol. Sci. 2012, 13(3), 3979-3987; doi:10.3390/ijms13033979
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Potentized homeopathic drug Arsenicum Album 30C inhibits intracellular reactive oxygen species generation and up-regulates expression of arsenic resistance gene in arsenite-exposed bacteria Escherichia coli
1. Arnab De (Cytogenetics and Molecular Biology Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, India )
2. Durba Das (Cytogenetics and Molecular Biology Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, India )
3. Suman Dutta (Cytogenetics and Molecular Biology Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, India )
4. Debrup Chakraborty (Cytogenetics and Molecular Biology Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, India )
5. Naoual Boujedaini (Boiron Laboratory, Lyon, France )
6. Anisur Rahman Khuda-Bukhsh (Cytogenetics and Molecular Biology Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, India)
OBJECTIVE:
To examine if potentized homeopathic drug Arsenicum Album 30C (Ars Alb 30C) can reduce sodium arsenite-induced toxicity in Escherichia coli.
METHODS:
E. coli were exposed to low arsenite insult after they grew up to log phase in standard Luria-Bertani medium. E. coli were treated with 1 or 2 mmol/L sodium arsenite alone (control), or Ars Alb 30C was added to the medium of a subset of sodium arsenite-treated bacteria (drug-treated), or homeopathically agitated alcohol was added to the medium containing a subset of sodium arsenite-treated bacteria (placebo-treated). A sub-set of untreated E. coli served as the negative control. Glucose uptake, specific activities of hexokinase, lipid peroxidase (LPO), superoxide dismutase (SOD) and catalase, intra- and extra-cellular sodium arsenite content, cell growth, cell membrane potential, DNA damage, intracellular reactive oxygen species (ROS), adenosine triphosphate (ATP) and free glutathione content and expressions of arsB and ptsG gene in normal control, sodium arsenite-treated, drug-treated and placebo-treated E. coli were analyzed. Treatments were blinded and randomized.
RESULTS:
In sodium arsenite-treated E. coli, glucose uptake, intracellular ROS, LPO and DNA damage increased along with decrease in the specific activities of hexokinase, SOD and catalase, intracellular ATP and free glutathione contents and cell membrane potential and growth, and there were increases in expression levels of arsB gene and ptsG gene. Ars Alb 30C administration reduced arsenic toxicity in E. coli by inhibiting generation of ROS and increasing tolerance to arsenite toxicity and cell growth.
CONCLUSION:
Ars Alb 30C ameliorated arsenic toxicity and DNA damage, validating efficacy of ultra-highly diluted remedies used in homeopathy.
Source : Journal Chinese Integrative Medicine
Link to Full Article
An initial report on the efficacy of a millesimal potency Arsenicum Album LM 0/3 in ameliorating arsenic toxicity in humans living in a high-risk arsenic village
1. Anisur Rahman Khuda-Bukhsh (Department of Zoology, Laboratory of Cytogenetics and Molecular Biology, University of Kalyani, Kalyani 741235, India
2. Antara Banerjee (Department of Zoology, Laboratory of Cytogenetics and Molecular Biology, University of Kalyani, Kalyani 741235, India )
3. Surjyo Jyoti Biswas (Department of Zoology, Laboratory of Cytogenetics and Molecular Biology, University of Kalyani, Kalyani 741235, India )
4. Susanta Roy Karmakar (Department of Zoology, Laboratory of Cytogenetics and Molecular Biology, University of Kalyani, Kalyani 741235, India )
5. Pathikrit Banerjee (Department of Zoology, Laboratory of Cytogenetics and Molecular Biology, University of Kalyani, Kalyani 741235, India )
6. Surajit Pathak (Department of Zoology, Laboratory of Cytogenetics and Molecular Biology, University of Kalyani, Kalyani 741235, India )
7. Bibhas Guha (Department of Zoology, Laboratory of Cytogenetics and Molecular Biology, University of Kalyani, Kalyani 741235, India )
8. Saiful Haque (Department of Zoology, Laboratory of Cytogenetics and Molecular Biology, University of Kalyani, Kalyani 741235, India )
9. Debarsi Das (Department of Zoology, Laboratory of Cytogenetics and Molecular Biology, University of Kalyani, Kalyani 741235, India )
10. Arnab De (Department of Zoology, Laboratory of Cytogenetics and Molecular Biology, University of Kalyani, Kalyani 741235, India )
11. Durba Das (Department of Zoology, Laboratory of Cytogenetics and Molecular Biology, University of Kalyani, Kalyani 741235, India )
12. Naoual Boujedaini (Boiron Laboratory, 20 rue de la Liberation, Sainte-Les-Foy-Lyon, Lyon, France )
Background:
Millions of people are at risk of groundwater arsenic contamination, and there is no known remedy that can effectively remove the symptoms of prolonged arsenic poisoning. A potentized homeopathic drug, Arsenicum Album LM 0/3 (Ars Alb LM 0/3), is claimed in homeopathic literature to have the ability to treat symptoms similar to that of arsenic poisoning.
Objective:
This study examines whether Ars Alb LM 0/3 could provide some degree of amelioration for the victims living in an arsenic-affected village where no arsenic-free drinking water is available.
Design, setting, participants and interventions:
This study was carried out on volunteers living in an arsenic-affected village where no arsenic-free drinking water is available. Twenty-eight volunteers from the village of Dasdiya, in Haringhata block under Nadia District, West Bengal, India, an arsenic-contaminated village where wells contain 55 to 95 μg/L arsenic, were selected to undertake a double-blind and placebo-controlled trial. The subjects provided samples of blood and urine before and after 2 months of taking either "verum" or "placebo". Another 18 subjects living in an arsenic-free village, served as the negative controls.
Main outcome measures:
Samples of blood and urine from the subjects were assayed for arsenic content, according to various toxicity biomarkers and pathophysiological parameters.
Results:
Out of the original 28 subjects, only 14 subjects provided samples while the other 14 dropped out. There were elevated levels of arsenic in the blood and urine, alkaline and acid phosphatases, lipid peroxidation, and glutathione activities and increased blood glucose, triacylglycerol, cholesterol, and low-density lipoprotein cholesterol contents, whereas there were decreased levels of aspartate and alanine aminotransferases, gamma glutamyl transferase, glucose-6-phosphate dehydrogenase contents, high-density lipoprotein cholesterol and packed cell volume in the subjects. After 2 months of homeopathic remedy administration, the verum-fed subjects showed positive modulations within these parameters with slight lowering of matrix metalloproteinase activity as compared with the placebo group.
Conclusion:
Ars Alb LM 0/3 shows potential for use in high-risk arsenic villages as an interim treatment for amelioration of arsenic toxicity until more extensive medical treatment and facilities can be provided to the numerous victims of arsenic poisoning.
Source : Chinese Journal of Integrative Medicine
Link to full article
Potentized homeopathic drug Arsenicum Album 30C positively modulates protein biomarkers and gene expressions in Saccharomyces cerevisae exposed to arsenate
1. Durba Das (Cytogenetics and Molecular Biology Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, India )
2. Arnab De (Cytogenetics and Molecular Biology Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, India )
3. Suman Dutta (Cytogenetics and Molecular Biology Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, India )
4. Raktim Biswas (Cytogenetics and Molecular Biology Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, India )
5. Naoual Boujedaini (Boiron Laboratory, Lyon, France )
6. Anisur Rahman Khuda-Bukhsh (Cytogenetics and Molecular Biology Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, India
Abstract
Objective:
This study examines if homeopathic drug Arsenicum Album 30C (Ars Alb 30C) can elicit ameliorative responses in yeast (Saccharomyces cerevisiae) exposed to arsenate.
Methods:
The yeast S. cerevisiae 699 was cultured in a standard yeast extract peptone dextrose broth medium. It was exposed to the final concentration of 0.15 mmol/L arsenate for two intervals, 1 h and 2 h, respectively. The cell viability was determined along with the assessment of several toxicity biomarkers such as catalase (CAT), superoxide dismutase (SOD), total thiol (GSH) and glucose-6-phosphate dehydrogenase (G6PDH), lipid peroxidation, protein carbonylation and DNA damage. Reactive oxygen species (ROS) accumulation, expressions of relevant stress transcription activators like Yap-1 and Msn 2, and mRNA expression of yeast caspase-1 (Yca-1) were also measured.
Results:
Treatment of arsenate increased lipid peroxidation, protein carbonylation, DNA damage, ROS accumulation and expressions of Yap-1, Msn 2 and Yca-1 and decreased GSH, G6PDH, CAT and SOD. Ars Alb 30C administration decreased lipid peroxidation, protein carbonylation, DNA damage, ROS formation and Msn 2 and Yca-1 expressions and increased cell viability, GSH, G6PDH, CAT and SOD significantly (P<0.05), except for a slight increase in Yap-1 expression.
Conclusion:
Ars Alb 30C triggers ameliorative responses in S. cerevisiae exposed to arsenate
Source : Journal of Chinese Integrative Medicine
Link to Full Article
1. Arnab De (Cytogenetics and Molecular Biology Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, India )
2. Durba Das (Cytogenetics and Molecular Biology Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, India )
3. Suman Dutta (Cytogenetics and Molecular Biology Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, India )
4. Debrup Chakraborty (Cytogenetics and Molecular Biology Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, India )
5. Naoual Boujedaini (Boiron Laboratory, Lyon, France )
6. Anisur Rahman Khuda-Bukhsh (Cytogenetics and Molecular Biology Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, India)
OBJECTIVE:
To examine if potentized homeopathic drug Arsenicum Album 30C (Ars Alb 30C) can reduce sodium arsenite-induced toxicity in Escherichia coli.
METHODS:
E. coli were exposed to low arsenite insult after they grew up to log phase in standard Luria-Bertani medium. E. coli were treated with 1 or 2 mmol/L sodium arsenite alone (control), or Ars Alb 30C was added to the medium of a subset of sodium arsenite-treated bacteria (drug-treated), or homeopathically agitated alcohol was added to the medium containing a subset of sodium arsenite-treated bacteria (placebo-treated). A sub-set of untreated E. coli served as the negative control. Glucose uptake, specific activities of hexokinase, lipid peroxidase (LPO), superoxide dismutase (SOD) and catalase, intra- and extra-cellular sodium arsenite content, cell growth, cell membrane potential, DNA damage, intracellular reactive oxygen species (ROS), adenosine triphosphate (ATP) and free glutathione content and expressions of arsB and ptsG gene in normal control, sodium arsenite-treated, drug-treated and placebo-treated E. coli were analyzed. Treatments were blinded and randomized.
RESULTS:
In sodium arsenite-treated E. coli, glucose uptake, intracellular ROS, LPO and DNA damage increased along with decrease in the specific activities of hexokinase, SOD and catalase, intracellular ATP and free glutathione contents and cell membrane potential and growth, and there were increases in expression levels of arsB gene and ptsG gene. Ars Alb 30C administration reduced arsenic toxicity in E. coli by inhibiting generation of ROS and increasing tolerance to arsenite toxicity and cell growth.
CONCLUSION:
Ars Alb 30C ameliorated arsenic toxicity and DNA damage, validating efficacy of ultra-highly diluted remedies used in homeopathy.
Source : Journal Chinese Integrative Medicine
Link to Full Article
An initial report on the efficacy of a millesimal potency Arsenicum Album LM 0/3 in ameliorating arsenic toxicity in humans living in a high-risk arsenic village
1. Anisur Rahman Khuda-Bukhsh (Department of Zoology, Laboratory of Cytogenetics and Molecular Biology, University of Kalyani, Kalyani 741235, India
2. Antara Banerjee (Department of Zoology, Laboratory of Cytogenetics and Molecular Biology, University of Kalyani, Kalyani 741235, India )
3. Surjyo Jyoti Biswas (Department of Zoology, Laboratory of Cytogenetics and Molecular Biology, University of Kalyani, Kalyani 741235, India )
4. Susanta Roy Karmakar (Department of Zoology, Laboratory of Cytogenetics and Molecular Biology, University of Kalyani, Kalyani 741235, India )
5. Pathikrit Banerjee (Department of Zoology, Laboratory of Cytogenetics and Molecular Biology, University of Kalyani, Kalyani 741235, India )
6. Surajit Pathak (Department of Zoology, Laboratory of Cytogenetics and Molecular Biology, University of Kalyani, Kalyani 741235, India )
7. Bibhas Guha (Department of Zoology, Laboratory of Cytogenetics and Molecular Biology, University of Kalyani, Kalyani 741235, India )
8. Saiful Haque (Department of Zoology, Laboratory of Cytogenetics and Molecular Biology, University of Kalyani, Kalyani 741235, India )
9. Debarsi Das (Department of Zoology, Laboratory of Cytogenetics and Molecular Biology, University of Kalyani, Kalyani 741235, India )
10. Arnab De (Department of Zoology, Laboratory of Cytogenetics and Molecular Biology, University of Kalyani, Kalyani 741235, India )
11. Durba Das (Department of Zoology, Laboratory of Cytogenetics and Molecular Biology, University of Kalyani, Kalyani 741235, India )
12. Naoual Boujedaini (Boiron Laboratory, 20 rue de la Liberation, Sainte-Les-Foy-Lyon, Lyon, France )
Background:
Millions of people are at risk of groundwater arsenic contamination, and there is no known remedy that can effectively remove the symptoms of prolonged arsenic poisoning. A potentized homeopathic drug, Arsenicum Album LM 0/3 (Ars Alb LM 0/3), is claimed in homeopathic literature to have the ability to treat symptoms similar to that of arsenic poisoning.
Objective:
This study examines whether Ars Alb LM 0/3 could provide some degree of amelioration for the victims living in an arsenic-affected village where no arsenic-free drinking water is available.
Design, setting, participants and interventions:
This study was carried out on volunteers living in an arsenic-affected village where no arsenic-free drinking water is available. Twenty-eight volunteers from the village of Dasdiya, in Haringhata block under Nadia District, West Bengal, India, an arsenic-contaminated village where wells contain 55 to 95 μg/L arsenic, were selected to undertake a double-blind and placebo-controlled trial. The subjects provided samples of blood and urine before and after 2 months of taking either "verum" or "placebo". Another 18 subjects living in an arsenic-free village, served as the negative controls.
Main outcome measures:
Samples of blood and urine from the subjects were assayed for arsenic content, according to various toxicity biomarkers and pathophysiological parameters.
Results:
Out of the original 28 subjects, only 14 subjects provided samples while the other 14 dropped out. There were elevated levels of arsenic in the blood and urine, alkaline and acid phosphatases, lipid peroxidation, and glutathione activities and increased blood glucose, triacylglycerol, cholesterol, and low-density lipoprotein cholesterol contents, whereas there were decreased levels of aspartate and alanine aminotransferases, gamma glutamyl transferase, glucose-6-phosphate dehydrogenase contents, high-density lipoprotein cholesterol and packed cell volume in the subjects. After 2 months of homeopathic remedy administration, the verum-fed subjects showed positive modulations within these parameters with slight lowering of matrix metalloproteinase activity as compared with the placebo group.
Conclusion:
Ars Alb LM 0/3 shows potential for use in high-risk arsenic villages as an interim treatment for amelioration of arsenic toxicity until more extensive medical treatment and facilities can be provided to the numerous victims of arsenic poisoning.
Source : Chinese Journal of Integrative Medicine
Link to full article
Potentized homeopathic drug Arsenicum Album 30C positively modulates protein biomarkers and gene expressions in Saccharomyces cerevisae exposed to arsenate
1. Durba Das (Cytogenetics and Molecular Biology Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, India )
2. Arnab De (Cytogenetics and Molecular Biology Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, India )
3. Suman Dutta (Cytogenetics and Molecular Biology Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, India )
4. Raktim Biswas (Cytogenetics and Molecular Biology Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, India )
5. Naoual Boujedaini (Boiron Laboratory, Lyon, France )
6. Anisur Rahman Khuda-Bukhsh (Cytogenetics and Molecular Biology Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, India
Abstract
Objective:
This study examines if homeopathic drug Arsenicum Album 30C (Ars Alb 30C) can elicit ameliorative responses in yeast (Saccharomyces cerevisiae) exposed to arsenate.
Methods:
The yeast S. cerevisiae 699 was cultured in a standard yeast extract peptone dextrose broth medium. It was exposed to the final concentration of 0.15 mmol/L arsenate for two intervals, 1 h and 2 h, respectively. The cell viability was determined along with the assessment of several toxicity biomarkers such as catalase (CAT), superoxide dismutase (SOD), total thiol (GSH) and glucose-6-phosphate dehydrogenase (G6PDH), lipid peroxidation, protein carbonylation and DNA damage. Reactive oxygen species (ROS) accumulation, expressions of relevant stress transcription activators like Yap-1 and Msn 2, and mRNA expression of yeast caspase-1 (Yca-1) were also measured.
Results:
Treatment of arsenate increased lipid peroxidation, protein carbonylation, DNA damage, ROS accumulation and expressions of Yap-1, Msn 2 and Yca-1 and decreased GSH, G6PDH, CAT and SOD. Ars Alb 30C administration decreased lipid peroxidation, protein carbonylation, DNA damage, ROS formation and Msn 2 and Yca-1 expressions and increased cell viability, GSH, G6PDH, CAT and SOD significantly (P<0.05), except for a slight increase in Yap-1 expression.
Conclusion:
Ars Alb 30C triggers ameliorative responses in S. cerevisiae exposed to arsenate
Source : Journal of Chinese Integrative Medicine
Link to Full Article