Research - Zincum metallicum
Protective effect of Zincum metallicum on rat model of Parkinson's disease
Andleeb Khan1, Gulrana Khuwaja2, Farah Islam2, Hayat Javed1, Tauheed Ishrat1, Kumar Vaibhav1, Badaruzaman Khan1, Rizwana Tabassum1, Minakshi Das1, Md Ejaz Ahmed1, Fakhrul Islam3
1 Department of Medical Elementology and Toxicology, Neurotoxicology Lab, (A FIST-DST and SAP-UGC Funded Department), New Delhi, India
2 Department of Biotechnology, Faculty of Pharmacy, Jamia Hamdard, Hamdard University, Hamdard Nagar, New Delhi, India
3 Department of Medical Elementology and Toxicology, Neurotoxicology Lab, (A FIST-DST and SAP-UGC Funded Department), New Delhi,
Abstract
Background: Parkinson's Disease (PD) is one of the major neurodegenerative disorders, and oxidative stress has been implicated in playing an important role in the pathogenesis of the disease. Zincum metallicum, produces symptoms mentioned in Homoeopathic Materia Medica which are akin to PD on which basis it might be considered as one at the intermediate to treat the disease.
Materials and Methods: Rats were divided into eight groups; surgery was done by stereotaxic apparatus. 6 - hydroxydopamine was used to induce parkinsonism thereafter on 16 th day of lesioning animals were assessed by the video path analyzer. Animals were sacrificed and biochemical assays (Lipid peroxidation [LPO], glutathione [GSH], glutathione peroxide [GPx], glutathione reductase [GR], glutathione-S-transferase [GST]) and level of dopamine (DA), 3,4-dihydroxyphenylacetic acid (DOPAC), and homovanillic acid (HVA), were estimated. Further dopaminergic D 2 receptor binding was also done to confirm the induced parkinsonism.
Results: The behavior activities (locomotor, distance travel, stereroevent) were decreased whereas the rest time was increased in lesion group animals as compared to the sham group. The locomotor activity and the distance traveled were protected significantly with 6C whereas rest time was protected significantly with 30C and 200C of Homoeopathic medicine Zincum metallicum. On the other hand, S + 30C and S + 200C groups have shown increased locomotor activities as compared to S group. The rest time was also increased significantly in S + 6C and S + 30C group animals as compared to S group. The elevated level of LPO and DA D 2 receptor binding density in PD group was protected significantly with Zincum metallicum (6C, 30C, and 200C). The depleted level of GSH and activity of antioxidant enzymes (GPx, GR, and GST) and DA and its metabolites DOPAC and HVA were protected significantly with Zincum metallicum (6C, 30C, and 200C).
Conclusion: The study indicates the Zincum metallicum may be helpful in slowing down injury in parkinsonism and could be a beneficial drug for the prevention of PD
Source : Indian Journal of Research in Homeopathy
Link to Full Article
ANTIOXIDANTE EFFECT IN VITRO OF THE HOMEOPATHIC MEDICINE ARSENICUM ALBUM, CUPRUM METALLICUM MANGANUM AND ZINCUM METALLICUM.
CELSO FERNANDES BATELLO
Dissertation presented as part of the demanded requirements to obtain the title of MA in Homeopathy by FACIS – IBEHE
Abstract
This dissertation, with the support of a theoretical practical foundation, presents the scenario where is inserted the problematic in question; The Antioxidant Effect in vitro of Homeopathic Medicines Arsenicum album, Cuprum metallicum, Manganum and Zincum metallicum.
It is demonstrated in the first chapters, of theoretical bibliographical substantiation, the Homeopathy and Oligotherapy as therapeutics techniques, as well as the importance of oxidation phenomena for a better comprehension of the organic phenomena, mainly in the genesis of many diseases. It is also experimentally demonstrated the homeopathic medicines antioxidant action in different dilutions in comparison with melatonin in various concentrations over the lipidic peroxidation in homogenate of mice brains measured though the malondialdehyde dosage obtained through the absorbency technique.
For the analysis of the results the Kruskal-Wallys and Dunn´s Multiple Comparisons tests were realized, that revealed significant differences among the experimented groups.
It was verified a greater lipidic peroxidation inhibiting effect with melatonin 1M, followed by melatonin 0.5M, Cuprum metallicum C12, Cuprum Metallicum C30, Arsenicum album C30, melatonine 0.24M, Manganum C30 and Arsenicum album C12.
It was proved that the melatonin has an in vitro lipidic peroxidation inhibiting effect, and so being adopted as reference. However the new fact arises from the observation of the significant lipidic peroxidation inhibition obtained with the usage of Homeopathic medicines, sometimes with dilutions that supersed the Avogadro number, as in the cases of Cuprum metallicum C30, Arsenicum album C30 and Manganum C30 in decreasing action order.
This work calls the attention for the possibility of existence of an antioxidant mechanism action of homeopathic medicine different from the know cause effect relationship.
Source : Homeopathy International
Link to Full Dissertation
Andleeb Khan1, Gulrana Khuwaja2, Farah Islam2, Hayat Javed1, Tauheed Ishrat1, Kumar Vaibhav1, Badaruzaman Khan1, Rizwana Tabassum1, Minakshi Das1, Md Ejaz Ahmed1, Fakhrul Islam3
1 Department of Medical Elementology and Toxicology, Neurotoxicology Lab, (A FIST-DST and SAP-UGC Funded Department), New Delhi, India
2 Department of Biotechnology, Faculty of Pharmacy, Jamia Hamdard, Hamdard University, Hamdard Nagar, New Delhi, India
3 Department of Medical Elementology and Toxicology, Neurotoxicology Lab, (A FIST-DST and SAP-UGC Funded Department), New Delhi,
Abstract
Background: Parkinson's Disease (PD) is one of the major neurodegenerative disorders, and oxidative stress has been implicated in playing an important role in the pathogenesis of the disease. Zincum metallicum, produces symptoms mentioned in Homoeopathic Materia Medica which are akin to PD on which basis it might be considered as one at the intermediate to treat the disease.
Materials and Methods: Rats were divided into eight groups; surgery was done by stereotaxic apparatus. 6 - hydroxydopamine was used to induce parkinsonism thereafter on 16 th day of lesioning animals were assessed by the video path analyzer. Animals were sacrificed and biochemical assays (Lipid peroxidation [LPO], glutathione [GSH], glutathione peroxide [GPx], glutathione reductase [GR], glutathione-S-transferase [GST]) and level of dopamine (DA), 3,4-dihydroxyphenylacetic acid (DOPAC), and homovanillic acid (HVA), were estimated. Further dopaminergic D 2 receptor binding was also done to confirm the induced parkinsonism.
Results: The behavior activities (locomotor, distance travel, stereroevent) were decreased whereas the rest time was increased in lesion group animals as compared to the sham group. The locomotor activity and the distance traveled were protected significantly with 6C whereas rest time was protected significantly with 30C and 200C of Homoeopathic medicine Zincum metallicum. On the other hand, S + 30C and S + 200C groups have shown increased locomotor activities as compared to S group. The rest time was also increased significantly in S + 6C and S + 30C group animals as compared to S group. The elevated level of LPO and DA D 2 receptor binding density in PD group was protected significantly with Zincum metallicum (6C, 30C, and 200C). The depleted level of GSH and activity of antioxidant enzymes (GPx, GR, and GST) and DA and its metabolites DOPAC and HVA were protected significantly with Zincum metallicum (6C, 30C, and 200C).
Conclusion: The study indicates the Zincum metallicum may be helpful in slowing down injury in parkinsonism and could be a beneficial drug for the prevention of PD
Source : Indian Journal of Research in Homeopathy
Link to Full Article
ANTIOXIDANTE EFFECT IN VITRO OF THE HOMEOPATHIC MEDICINE ARSENICUM ALBUM, CUPRUM METALLICUM MANGANUM AND ZINCUM METALLICUM.
CELSO FERNANDES BATELLO
Dissertation presented as part of the demanded requirements to obtain the title of MA in Homeopathy by FACIS – IBEHE
Abstract
This dissertation, with the support of a theoretical practical foundation, presents the scenario where is inserted the problematic in question; The Antioxidant Effect in vitro of Homeopathic Medicines Arsenicum album, Cuprum metallicum, Manganum and Zincum metallicum.
It is demonstrated in the first chapters, of theoretical bibliographical substantiation, the Homeopathy and Oligotherapy as therapeutics techniques, as well as the importance of oxidation phenomena for a better comprehension of the organic phenomena, mainly in the genesis of many diseases. It is also experimentally demonstrated the homeopathic medicines antioxidant action in different dilutions in comparison with melatonin in various concentrations over the lipidic peroxidation in homogenate of mice brains measured though the malondialdehyde dosage obtained through the absorbency technique.
For the analysis of the results the Kruskal-Wallys and Dunn´s Multiple Comparisons tests were realized, that revealed significant differences among the experimented groups.
It was verified a greater lipidic peroxidation inhibiting effect with melatonin 1M, followed by melatonin 0.5M, Cuprum metallicum C12, Cuprum Metallicum C30, Arsenicum album C30, melatonine 0.24M, Manganum C30 and Arsenicum album C12.
It was proved that the melatonin has an in vitro lipidic peroxidation inhibiting effect, and so being adopted as reference. However the new fact arises from the observation of the significant lipidic peroxidation inhibition obtained with the usage of Homeopathic medicines, sometimes with dilutions that supersed the Avogadro number, as in the cases of Cuprum metallicum C30, Arsenicum album C30 and Manganum C30 in decreasing action order.
This work calls the attention for the possibility of existence of an antioxidant mechanism action of homeopathic medicine different from the know cause effect relationship.
Source : Homeopathy International
Link to Full Dissertation